Fuhrer
Fakultäten » Medizinische Fakultät » Hirnforschung, Institut für » Prof. Dr. Christian Fuhrer » Fuhrer
Completed research project
| Title / Titel | Postsynaptic assembly induced by agrin and laminin | ||
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| Abstract (PDF, 14 KB) | |||
| Summary / Zusammenfassung | Clustering of acetylcholine receptors (AChRs) in the muscle membrane is one of the first signs of postsynaptic differentiation during formation of the neuromuscular junction and can be induced by components of the basal lamina such as agrin and laminin. We analyze how other postsynaptic proteins are redistributed by these two components, how they interact with AChRs to cause clustering, and what role AChRs themselves play in this process. The current data indicate that laminin and agrin act by different yet related pathways, as some postsynaptic proteins are differentially distributed after treatment of muscle cells with either factor, whereas tyrosine kinases and the scaffolding protein rapsyn are required in both cases. Furthermore, muscle cells were found to contain pre-assembled complexes of AChRs in association with other postsynaptic proteins, and such complexes play a role in postsynaptic assembly induced by agrin. Weitere Informationen |
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| Publications / Publikationen | Cossins, J.A., Burke, G., Maxwell, S., Spearman, H., Man, S., Kuks, J., Vincent, A., Palace, J., Fuhrer, C., and Beeson, D. 2006. Diverse molecular mechanisms involved in AChR deficiency due to rapsyn mutations. Brain 129: 2773-2783.Burden, S.J., Fuhrer, C. and Hubbard, S.R.(2003). Agrin / MuSK signalling: willing and Abl. Nat Neurosci 6: 653-654.Banks, G.B., Fuhrer, C., Adams, M.E. and Froehner, S.C. (2003). The postsynaptic submembrane machinery at the neuromuscular junction: requirement for rapsyn and the utrophin/dystrophin-associated complex. J Neurocytol 32: 709-726.Moransard, M., Borges, L.S., Willmann, R., Marangi, P.A., Brenner, H.R., Ferns, M.J., and Fuhrer, C. (2003). Agrin regulates rapsyn interaction with surface AChRs which underlies cytoskeletal anchoring and clustering. J Biol Chem: 278. 7350-7359.Marangi, P.A., Wieland, S.T. and Fuhrer, C. (2002). Laminin-1 redistributes postsynaptic proteins and requires rapsyn, tyrosine phosphorylation, and Src and Fyn to stably cluster acetylcholine receptors. J Cell Biol 157: 883-895.Marangi, P.A., Forsayeth, J.R., Mittaud, P., Erb-Vögtli, S., Blake, D.J., Moransard, M., Sander, A., and Fuhrer, C. (2001). Acetylcholine receptors are required for agrin-induced clustering of postsynaptic proteins. EMBO J 20: 7060-7073.Weitere Informationen | ||
| Keywords / Suchbegriffe | Neurosciences | ||
| Project leadership and contacts / Projektleitung und Kontakte |
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| Other links to external web pages | http://www.neuroscience.uzh.ch/e/groups/fuhrer00.html | ||
| Funding source(s) / Unterstützt durch |
Universität Zürich (position pursuing an academic career), SNF (Personen- und Projektförderung) Swiss Foundation for Research on Muscle Diseases |
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| Duration of Project / Projektdauer | Oct 1998 to Dec 2006 |