Completed Research Project

Title / Titel
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Identification of proteins that functionally interact with neuronal nicotinic acetylcholine receptors
Summary / Zusammenfassung
Nicotinic receptors are expressed throughout the peripheral and central nervous system. Some receptors, such as alpha7, are found not only in presynaptic terminals, regulating the release of neurotransmittors, but also at postsynaptic sites mediating fast synaptic transmission. We use the yeast two hybrid assay and biochemical methods to identify proteins that interact with and regulate neuronal nicotinic receptors. In neuroblastoma cells, stably transfected human alpha7 nicotinic receptors were associated with Src-family kinases and phosphorylated by these kinases. Tyrosine phosphorylation and Src kinases negatively regulated the receptors in oocytes and neurons. We identified a first scaffold protein of the alpha 7 receptors. It colocalized with the receptors in clusters in neurons and downregulated aggregation of the alpha 7 receptors at the surface.
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Publications / Publikationen
Wiesner, A., and Fuhrer, C. 2006. Regulation of nicotinic receptors by tyrosine phosphorylation in the peripheral and central nervous system: same players, different roles. Cell. Mol. Life Sci. 63: 2818-2828.

Charpantier, E., Wiesner, A., Huh, K.-H., Ogier, R., Hoda, J.-C., Allaman, G., Raggenbass, M., Feuerbach, D., Bertrand, D., and Fuhrer, C. 2005. Alpha 7 neuronal nicotinic acetylcholine receptors are negatively regulated by tyrosine phosphorylation and Src-family kinases. J. Neurosci. 25: 9836-9849.

Huh, K.-H. and Fuhrer, C. (2002). Clustering of nicotinic acetylcholine receptors: from the neuromuscular junction to interneuronal synapses. Mol Neurobiol 25: 79-112.

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Keywords / Suchbegriffe
Neurosciences
Project Leadership and Contacts /
Projektleitung und Kontakte
Prof. Christian Fuhrer (Project Leader)chfuhrer@hifo.uzh.ch
Other Links to external Webpages
http://www.neuroscience.uzh.ch/e/groups/fuhrer00.html
Funding Source(s) /
Unterstützt durch
Universität Zürich (position pursuing an academic career), SNF (Personen- und Projektförderung)
 
In Collaboration with /
In Zusammenarbeit mit
Dr. Dominik Feuerbach
Novartis Pharma AG
Basel		Switzerland

Prof. Daniel Bertrand
University of Geneva

Switzerland

Duration of Project / Projektdauer
Oct 1998 to Dec 2008

© Universität Zürich, 1. Januar 2009, Impressum